Overview of the Role of Chromatographic Modes in Pharmaceutical Peptide Analysis
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Abstract
Peptides represent promising candidates for medical treatments. They can be designed for high specificity, which minimizes the adverse effects. Ensuring their purity, identity, and quality is crucial to their therapeutic efficacity. Peptides exhibit different physical and chemical properties (such as charge, pI, hydrophobic nature, size, etc.). These characteristics complicate the separation of a mixture of peptides. Various analytical techniques were used for peptide purification, peptide mapping, or peptide identification. HPLC continues to be the preferred method for analyzing peptides. Different HPLC modes were applied to separate peptides from their impurities and related substances. Among these modes, ion exchange, reversed-phase, normal-phase, and HILIC were performed to achieve peptide separation. This review will discuss chromatographic techniques and their role in peptide analysis, including analyzing peptide mixtures, creating peptide mapping, or isolating the peptide of interest from associated compounds.
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D'Addio, S. M., Bothe, J. R., Neri, C., Walsh, P. L., Zhang, J., Pierson, E., Mao, Y., Gindy, M., Leone, A., & Templeton, A. C. (2016). New and Evolving Techniques for the Characterization of Peptide Therapeutics. Journal of pharmaceutical sciences, 105(10), 2989–3006. DOI: https://doi.org/10.1016/j.xphs.2016.06.011.
Isbera, M., Abbood, A., & Ibrahim, W. (2016). Weight and Content Uniformity of Warfarin Sodium Half Tablets. Research Journal of Pharmacy and Technology, 9(3):215-218. DOI: https://doi.org/10.5958/0974-360X.2016.00039.1.
Abbood, A., & Layka, R. (2017). Weight and content uniformity Study of captopril half-tablets. Research Journal of Pharmacy and Technology, 10(6):1621-1626. DOI: https://doi.org/10.5958/0974-360X.2017.00285.2.
Chbani D, Abbood A, & Alkhayer M. (2018). Determination of Nitrite and Nitrate Ions levels in some types of processed meats marketed locally. Research Journal of Pharmacy and Technology, 11(4):1442-1447. DOI: https://doi.org/10.5958/0974-360X.2018.00269.X.
Abbood, A., Malek, Z., Al-Homsh, Y, & Thallaj, N. (2022). In vitro Study for Antibiotic resistance of bacteria causing Urinary Tract Infection from Syrian adults. Research Journal of Pharmacy and Technology, 15(10):4727-2. DOI: https://doi.org/10.52711/0974-360X.2022.00794.
Abbood, A., Malek, Z., & Thallaj, N. (2022). Antibiotic resistance of urinary tract pathogens in Syrian children. Research Journal of Pharmacy and Technology, 15(11):4935-9. DOI: https://doi.org/10.52711/0974-360X.2022.00829.
Abbood, A. (2018). Determination of phenolic content and antioxidant activity of some cosmetic creams available in the Syrian market. Journal of Chemical and Pharmaceutical Sciences, 11:280-3. DOI: https://doi.org/10.30558/jchps.20181104006.
Zrekah, G.H., Diab, D.A., Abboud, A. (2016). Determination of Protein and fat oxidation levels in imported infant formula available in Syria. International Journal of Pharmacy and Pharmaceutical Sciences, 8:169-72. https://journals.innovareacademics.in/index.php/ijpps/article/view/9899.
Lynch, K. B., Ren, J., Beckner, M. A., He, C., & Liu, S. (2019). Monolith columns for liquid chromatographic separations of intact proteins: A review of recent advances and applications. Analytica chimica acta, 1046, 48–68. DOI: https://doi.org/10.1016/j.aca.2018.09.021.
Abbood, A. (2023). Optimization of the Imaged cIEF Method for Monitoring the Charge Heterogeneity of Antibody-Maytansine Conjugate, Journal of Analytical Methods in Chemistry, Article ID 8150143, 10 pages. DOI: https://doi.org/10.1155/2023/8150143.
Abbood, A. (2024). Study of formulation effects on the charge variant profile of antibody-maytansine conjugates by icIEF method. Acta Pharm. Sci, 62 (2): 288-300. https://www.actapharmsci.com/abstract.php?id=872.
Gu, B., Chen, Z., Thulin, C.D., & Lee, M.L. (2006). Efficient Polymer Monolith for Strong Cation-Exchange Capillary Liquid Chromatography of Peptides. Analytical Chemistry, 78(11). DOI: https://doi.org/10.1021/ac060284r.
Chen ,X., Tolley, H.D., Lee, M.L. (2009). Polymeric strong cation-exchange monolithic column for capillary liquid chromatography of peptides and proteins. J Sep Sci, 32(15-16):2565-2573. DOI: https://doi.org/10.1002/jssc.200900255.
Thallaj, N. (2024). Advancements in Pharmaceutical Science: Synthesis and Application of Molecular Cages Integrating N-Heterocyclic Carbenes for Enhanced Stability and Functionality. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-5 Issue-1, pages 6-19. DOI: https://doi.org/10.54105/ijapsr.A4063.05011224.
Alrasho, J.F, Sofi, F.K., & Thallaj, N. (2024). Advancements in Antiviral Therapeutics: A Comprehensive Review of Hepatitis C Virus and Novel Flavone Leads. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-5 Issue-1, pages 28-40. DOI: https://doi.org/ 10.54105/ijapsr.A4064.05011224.
Abbood, A. (2024). Monitoring the charge variant profile of antibody-tomaymycin conjugates by icIEF method, Acta Pharm. Sci, 62 (1), 226-239. https://actapharmsci.com/uploads/pdf/pdf_868.pdf
Abbood, A., Herrenknecht, C., Proczek, G., Descroix, S., Rodrigo, J., Taverna, M., & Smadja, C. (2011). Hexylacrylate-based mixed-mode monolith, a stationary phase for the nano-HPLC separation of structurally related enkephalins. Analytical and bioanalytical chemistry, 400(2), 459–468. DOI: https://doi.org/10.1007/s00216-011-4762-4.
Kumar, V., Barwal, A., Sharma, N., Mir, D. S., Kumar, P., & Kumar, V. (2024). Therapeutic proteins: developments, progress, challenges, and future perspectives. 3 Biotech, 14(4), 112. DOI: https://doi.org/10.1007/s13205-024-03958-z
Asaad, R.A. & Abdullah, S.S. (2018). Breast Cancer Subtypes (BCSs) Classification according to Hormone Receptor Status: Identification of Patients at High Risk in Jableh- Syria. Research Research Journal of Pharmacy and Technology, 11(8): 3703-3710. DOI: https://doi.org/10.5958/0974-360X.2018.00680.7.
Asaad, R.A. (2017). Hormone Receptor Status and its Relation to C-Reactive Protein and other Prognostic factors in Breast Cancer in Jableh- Syria. Research Journal of Pharmacy and Technology, 10(9):3003-3010. DOI: https://doi.org/ 10.5958/0974-360X.2017.00532.7.
Labban, L., & Thallaj, N. (2019). The Effect of Magnesium Supplementation on Hba1c Level and Lipid Profile Among Type 2 Diabetics. Acta Scientific Nutritional Health, 3,10, 7-12. DOI: https://doi.org/10.31080/ASNH.2019.03.0435.
Labban, L., Thallaj, N., & Malek, Z. (2019). The implications of E-cigarettes or" vaping" on the nutritional status. Journal of Medical Research and Health Sciences, 2, 11, 784-787. https://www.semanticscholar.org/paper/The-implications-of-E-cigarettes-or-%22vaping%22on-the-Labban-Thallaj/bed5ffcf44abca8771c3fb597b71f7497f0b2ca5
Labban, L., Thallaj, N., & Labban, A. (2020). Assessing the Level of Awareness and Knowledge of COVID-19 Pandemic among Syrians. Archives of Medicine, 12, 2:8, 1-5. https://www.researchgate.net/publication/342677705_Assessing_the_Level_of_Awareness_and_Knowledge_of_COVID_19_Pandemic_among_Syrians
Mant, C.T., & Hodges, R.S. (1985). Separation of peptides by strong cation-exchange high-performance liquid chromatography. J Chromatogr. 327:147-155. DOI: https://doi.org/10.1016/s0021-9673(01)81643-5.
Bouhallab, S., Henry, G., & Boschetti, E. (1996). Separation of small cationic bioactive peptides by strong ion-exchange chromatography.” Journal of Chromatography A, 724:137-145. DOI: https://doi.org/10.1016/0021-9673(95)00928-0.
Mant, C. T., Chen, Y., Yan, Z., Popa, T. V., Kovacs, J. M., Mills, J. B., Tripet, B. P., & Hodges, R. S. (2007). HPLC analysis and purification of peptides. Methods in molecular biology (Clifton, N.J.), 386, 3–55. DOI: https://doi.org/10.1007/978-1-59745-430-8_1
Sanz-Nebot, V., Benavente, F., Castillo, A., & Barbosa, J. (2000). Liquid chromatography-electrospray mass spectrometry of multicomponent peptide mixtures. Characterization of a mixture from the synthesis of the hormone goserelin. Journal of chromatography. A, 889(1-2), 119–133. DOI: https://doi.org/10.1016/s0021-9673(00)00394-0
Morkus, R., &Abbood, A. (2024). A Survey of the Awareness and Practices of Antibiotic Use Among College Undergraduates and Graduates in Latakia. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-4 Issue-3, pp: 1-6. DOI: https://doi.org/10.54105/ijapsr.C4039.04030424.
Gazdag, M., Mihályfi, K., & Görög, S. (1997). Ion-pair RP-HPLC separation of thymopoietin fragments and related peptides, Anal. Chim. Acta, 352, 231-237. DOI: https://doi.org/10.1016/S0003-2670(97)00119-0.
Shibue, M., Mant, C. T., & Hodges, R. S. (2005). Effect of anionic ion-pairing reagent concentration (1-60 mM) on reversed-phase liquid chromatography elution behaviour of peptides. Journal of chromatography. A, 1080(1), 58–67. DOI: https://doi.org/10.1016/j.chroma.2005.02.047.
Mant, C. T., Byars, A., Ankarlo, S., Jiang, Z., & Hodges, R. S. (2018). Separation of highly charged (+5 to +10) amphipathic α-helical peptide standards by cation-exchange and reversed-phase high-performance liquid chromatography. Journal of chromatography. A, 1574, 60–70. DOI: https://doi.org/10.1016/j.chroma.2018.09.003.
Machkour, A., Thallaj, N.K., Benhamou, L., Lachkar, M., & Mandon, D. (2006). The Coordination Chemistry of FeCl3 and FeCl2 to Bis [2‐(2, 3‐dihydroxyphenyl)‐6‐pyridylmethyl](2‐pyridylmethyl) amine: Access to a Diiron (iii) Compound with an Unusual Pentagonal‐Bipyramidal/Square‐Pyramidal Environment Chemistry–A European Journal, 25;12(25): 6660-6668. DOI: https://doi.org/10.1002/chem.200600276.
Kimura, H., Tanigawa, T., Morisaka, H., Ikegami, T., Hosoya, K., Ishizuka, N., Minakuchi, H., Nakanishi, K., Ueda, M., Cabrera, K., & Tanaka, N. (2004). Simple 2D-HPLC using a monolithic silica column for peptide separation. Journal of separation science, 27(10-11), 897–904. DOI: https://doi.org/10.1002/jssc.200401842.
Thallaj, N.K., Przybilla, J., Welter, R., & Mandon, D. (2008). A ferrous center as a reaction site for hydration of a nitrile group into a carboxamide in mild conditions. J. Am. Chem. Soc, 130, 2414-2415. DOI: https://doi.org/10.1021/ja710560g.
Thallaj, N. (2022). Microwave-Assisted Synthesis of Oxadiazole and Thiazolidine Derivatives. Indian Journal of Advanced Chemistry, 1, 3, 2022. 10-14. DOI: https://doi.org/10.54105/ijac.d2015.102222.
Thallaj, N. (2022). Quick Review of Chemistry Related to the [Fe]- Hydrogenases. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), 2,4, 1-15. DOI: https://doi.org/10.54105/ijapsr.C4016.062422.
Thallaj N. (2022). A Short Review of Some Examples of the Binding of Fullerenes C60 to Transition Metal Complexes. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), 2, 6, 1-12. DOI: https://doi.org/10.54105/ijapsr.C4015.102622.
Thallaj, N. (2023). Review of a Few Selected Examples of Intermolecular Dioxygenases Involving Molecular Oxygen and Non-Heme Iron Proteins. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR). 3, 2, 1-18. DOI: https://doi.org/10.54105/ijapsr.C4011.023223.
Thallaj, N. (2023). A Brief Overview of the General Characteristics and Reactivity Towards Dioxygen of the Ferrous Tris (2-Pyridylmethyl Amine) Series Complexes is Presented. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR). 3, 3, 1-18. DOI: https://doi.org/10.54105/ijapsr.C4012.043323.
Thallaj, N. (2024). Detecting Antioxidant Behavior for Phenolic Content of Some Beauty Care Creams
in Syrian Market. Indian Journal
of Advanced Chemistry, vol. 2,
no. 1, pp. 10–14.
DOI: https://doi.org/10.54105/ijac.C2013.041322.
Thallaj, N.K., Mandon, D., & White, K.A. (2007). The Design of Metal Chelates with a Biologically Related Redox‐Active Part: Conjugation of Riboflavin to Bis (2‐pyridylmethyl) amine Ligand and Preparation of a Ferric Complex Eur. J. of Inorg. Chem., 44–47. DOI: https://doi.org/10.1002/ejic.200600789.
Thallaj, N.K., Orain, P.Y., Thibon, A., Sandroni, M., Welter. R, & Mandon, D. (2014). Steric Congestion at, and Proximity to, a Ferrous Center Leads to Hydration of α-Nitrile Substituents Forming Coordinated Carboxamides. Inorg Chem, 4;53(15):7824-36. DOI: https://doi.org/10.1021/ic500096h.
Wane, A., Thallaj, N.K., & Mandon, D. (2008). The Reactivity of Molecular Dioxygen on a Series of Isostructural Dichloroferrous Complexes with Tripodal Tetraamine Ligands: General Access to µ-oxo Diferric Complexes, and Effect of α-Fluorination on the Kinetics of the Reaction. Chemistry A European Journal, 14 (22), 6742-6753. DOI: https://doi.org/10.1002/chem.200701967.
Besher, S., Alallan, L., Hasan, M.I., Alshamaa, I., & Thallaj, N. (2024). Influence of Soil Salinity on the Chemical Composition of Essential Oil of Rosmarinus officinalis in Syria. Research Journal of Pharmacy and Technology, 17(5):2282-8. DOI: http://doi.org/10.52711/0974-360X.2024.00358.
Khatib, O., Alshimale, T., Alsaadi, A., & Thallaj, N. (2024). The Global Impact of HIV: A Comprehensive Review. IJAPSR, vol. 4, no. 3, pp. 6–19,. DOI: http://doi.org/10.54105/ijapsr.C4040.04030424.
Salloum, R., Baddour, F., & Abbood, A. (2024). A Questionnaire to Evaluate Undergraduate Students’ Consumption and Awareness of Non-Steroidal Anti-Inflammatory Drugs in Syria. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-4 Issue-4, pages 1-6. DOI: https://doi.org/10.54105/ijapsr.C4041.04040624.
Zanboua, R., & Abbood, A. (2024). Survey of Knowledge About the Interaction Between Food and Drugs Among the Syrian Population. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-4 Issue-4, pages 22-28. DOI: https://doi.org/10.54105/ijapsr.D4044.04040624.
Mahfouz, H., Assaf, A., & Abbood, A. (2024). Survey of Usage and Awareness of Ibuprofen Among the Syrian Population. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-4 Issue-5, pages 23-28. DOI: https://doi.org/10.54105/ijapsr.E4048.04050824.
Antakly, R., Najjar, F., & Abbood, A. (2024). Statistical Overview of Drug Shortage in Syria. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-5 Issue-1, pages 1-5. DOI: https://doi.org/10.54105/ijapsr.A4059.05011224.
Abbood, A. (2024). Insights into Therapeutic Peptides and their Quality Control. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-5 Issue-1, pages 16-27. DOI: https://doi.org/10.54105/ijapsr.A4059.05011224.
Abbood A. Overview of Analytical Methods for Characterizing the Charge Heterogeneity of Antibody-Drug Conjugates. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-4 Issue-5, August 2024, pages 16-22. DOI: https://doi.org/10.54105/ijapsr.E4047.04050824.
Nouira, R., & Abbood, A. (2024). Assessment of Knowledge About High Blood Pressure Among Syrians. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-4 Issue-6, pages 28-32. DOI: https://doi.org/10.54105/ijapsr.F4053.04061024.
Malek, Z., Abbood, A., & Thallaj, N. (2022). "Xi'an ShiyouDaxueXuebao (ZiranKexue Ban)." Journal of Xi'an Shiyou University, Natural Sciences, 302-312. https://xianshiyoudaxuexuebao.com/dashboard/uploads/19.K56XY..pdf
Al-Saroukhy, R., Al-Kara, R., Habib, R., & Abbood, A. (2024). Assessment of use and Awareness of Diclofenac in Syria. International Journal of Advanced Pharmaceutical Sciences and Research (IJAPSR), Volume-4 Issue-6, pages 1-6. DOI: https://doi.org/10.54105/ijapsr.F4052.04061024.
Asaad, R.A. (2023). The association between Triglyceride-Glucose index and Hypertension status (stages and phenotypes) in Type II Diabetes Mellitus. Research Journal of Pharmacy and Technology, 16(6): 2963-2968. DOI: https://doi.org/10.52711/0974-360X.2023.00489
Asaad, R.A. (2022). Evaluation of adiposity phenotypes: lipid accumulation product index, visceral adipose index, and body roundness index as predictor markers for metabolic syndrome development in type 2 diabetes mellitus. Bulletin of Pharmaceutical Sciences. Assiut, 45(2): 097-1107. DOI: https://doi.org/10.21608/bfsa.2022.271823
Alpert, A.J. (1990). Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds. J Chromatogr, 499:177-196. DOI: https://doi.org/10.1016/s0021-9673(00)96972-3.
Asaad, R.A. (2018). Lymph Node Ratio (LNR) as a predictive factor in addition to pNstaging in Syrian-breast cancer patients at diagnosis. Research Journal of Pharmacy and Technology. 11(3):933-940. DOI: https://doi.org/10.5958/0974-360X.2018.00173.7
Asaad, R.A. (2023). Relative Fat Mass (RFM) Evaluates the Whole Body Fat (WBF) and predicts Cardio-metabolic Disorders as a new obesity marker in Syrian-population. Research Journal of Pharmacy and Technology, 16(9):4399-5. DOI: https://doi.org/10.52711/0974-360X.2023.00719.