HPLC Method Validation for Determination of Pentoxifylline in Pharmaceutical Dosage Forms
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Abstract
Selective and simple reversed phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for quantification of pentoxifylline in dosage forms available in local market. Firstly, different chromatographic conditions were tested. Then, the optimized method was validated. The method proven to be linear over 50% to 150% of the nominal concentration of standard pentoxifylline (R2 0.994). The method was accurate (recovery 100.1%) and precise (RSD% <3%). The method could separate pentoxifylline of caffeine and degradation products. The method was suitable for routine analysis of pentoxifylline in tablet dosage forms.
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George J, Abel P, Pentoxifylline, xPharm: The Comprehensive Pharmacology Reference, 2008, 1-18. [CrossRef]
Feret W, Nalewajska M, Wojczyński Ł, Witkiewicz W, Kłos P, Dziedziejko V, Pawlik A. Pentoxifylline as a Potential Adjuvant Therapy for COVID-19: Impeding the Burden of the Cytokine Storm. J Clin Med. 2021, Vol. 15, 5305. [CrossRef]
Monteiro J. P.، Alves M. G.، Oliveira P. F.، Silva B. M.، Structure- Bioactivity Relationships of Methylxanthines: Trying to Make Sense of All the Promises and the Drawbacks. Molecules، Vol 21، 2016، ، 974-1006. [CrossRef]
McCarty M. F.، O'Keefe J. H.، DiNicolantonio J. J.، Pentoxifylline for vascular health: a brief review of the literature. Open Heart. Vol. 3، 2016، doi: 10.1136/openhrt-2015-000365. [CrossRef]
Bauza M.T.، Smith R. V.، Knutson D. E.، Witter F. R.، Gas chromatographic determination of pentoxifylline and its major metabolites in human breast milk. J. Chromatogr. Vol. 310، 1984، 61-69. [CrossRef]
Burrows J. L.، Jolley K. W.، Determination of pentoxifylline and four metabolites in plasma and urine by automated capillary gas chromatography using nitrogen-selective detection. J. Chromatogr. Vol 344، 1985، 187-98. [CrossRef]
Smith R.V.، Yang S.K.، Davis P.J.، Bauza M.T.، Determination of pentoxifylline and its major metabolites in microbial extracts by thin-layer and high-performance liquid chromatography. J. Chromatogr. A .Vol. 281، 1983، 281–287. [CrossRef]
Grozdanovic O.، Antic D.، Agbaba D.، Development of a HPTLC
method for in-process purity testing of pentoxifylline. J. Sep. Sci. Vol. 28، 2005، 575–580. [CrossRef]
Rahmant B. M.، Islam M. A.، Wahed M. I.، Maruf Ahmed I.، Islam R.، Barman R. K.، Anisuzzaman A. S. M.، and Proma Khondkar، Invitro Studies of Pentoxifylline Controlled-Release from Hydrophilic Matrices. J. Sci. Res. Vol. 1، 2009، 353-362. [CrossRef]
Teresa G. P.، Charyulu N.R.، Harish N.M.، Vishalakshi B.، Design and In Vitro Evaluation of Pentoxifylline- Polyelectrolyte Complex Tablets. J. drug. deliv. ther. Vol. 3، 2013، 85-90.
Hegde R. N.، Nandibewoor S. T.، Electrochemical Oxidation of Pentoxifylline and its Analysis in Pure and Pharmaceutical Formulations at a Glassy Carbon Electrode. Analytical Letters. Vol. 41،. 6، 2008، 977-991. [CrossRef]
Abbar J. C.، Malode S. J.، Nandibewoor S. T.، Electrochemicalmdetermination of a hemorheologic drug، pentoxifylline at a multiwalled carbon nanotube paste electrode. Bioelectrochemistry. Vol. 83، 2012، 1-7. [CrossRef]
Korman M.، Vindevogel J.، Sandra P.، Application of micellar electrokinetic chromatography to the quality control of pharmaceutical formulations: the analysis of xanthine derivatives. Electrophoresis. Vol. 10، 1994، 1304-1309. [CrossRef]
Sripalakit P.، Saraphanchotiwitthaya A.، Validation of an HPLC method for determination of pentoxifylline in human plasma and its application to pharmacokinetic study. J. AOAC. Int. Vol. 92، 2009، 837-845. [CrossRef]
Chmielewska A.، Konieczna L.، Plenis A.، Lamparczyk H.، Quantitative determination of pentoxifylline in human plasma. ACTA Chromatogr. Vol. 16، 2006، 70-79.
Lahsini R.، Monser L.، Optimization and validation of a new HPLC method using monolithic column for simultaneous determination of pentoxifylline and related compounds. Pharm. Chem. J. Vol. 46، 2012، 127-131. [CrossRef]
Boyka G. Tsvetkova، Ivanka P. Pencheva، Plamen T. Peikov، Simultaneous determination of pentoxifylline and its impurities in tablet dosage forms by RP-HPLC. Der. Pharma. Chemica. Vol. 4، 2012، 608-612.
Korany M. A.، Haggag R. S.، Ragab M. A. A.، Elmallah O. A.، A validated stability indicating DAD–HPLC method for determination of pentoxifylline in presence of its pharmacopeial related substances. Bulletin of Faculty of Pharmacy، Cairo University. Vol. 51، 2013، 211–219. [CrossRef]
Machkour A, Thallaj NK, Benhamou L, Lachkar M, Mandon D.Chemistry. 2006 Aug 25;12(25):6660-8.P 6660-6661-6662-6663. [CrossRef]
Thallaj, N., Machkour, A., Mandon, D., Welter, R., New. J. Chem., 2005, 29, 1555 – 1558. [CrossRef]
Thallaj, N. K. Damascus University Journal for Basic Sciences. 34 (1) 2018.
Thallaj. N. K. Journal of AlBaath University (39) 2017.
Thallaj. N. K. Tishreen University Journal for Research and Scientific Studies 38 (6) 2016.
Thallaj NK, Rotthaus O, Benhamou L, Humbert N, Elhabiri M, Lachkar M, Welter R, Albrecht-Gary AM, Mandon D. Chemistry. 2008;14(22):6742-53.P6745-6746-6747. [CrossRef]
Wane A, Thallaj NK, Mandon D. Chemistry. 2009 Oct 12;15(40):10593-602. P10594-10595-10595. [CrossRef]
Thallaj NK, Orain PY, Thibon A, Sandroni M, Welter R, Mandon D. Inorg Chem. 2014 Aug 4;53(15):7824-36. P7826-7827-7828. [CrossRef]
N. K. Thallaj, J. Przybilla, R. Welter and D. Mandon, J. Am. Chem. Soc. 2008, 130, 2414-2415. [CrossRef]
N. K. Thallaj, D. Mandon and K. A. White, Eur. J. of Inorg. Chem., 2007, 44–47. [CrossRef]
N. K. Thallaj, A. Machkour, D. Mandon and R. Welter, New J. Chem., 2005, 29, 1555–1558. [CrossRef]
Thallaj, N.; International journal of applied chemistry and biological sciences 2021, 2 (4), 65-77.
Thallaj, N.; Indian Journal of Advanced Chemistry (IJAC)2021, 1 (2), . [CrossRef]
Thallaj, N.; International Journal of Research Publication and Reviews (IJRPR)2021, 2, 10, 951-959 [CrossRef]
L. Labban, N. Thallaj, Z. Malek; International Journal of Medical Studies, 2020, 5, No 12, 23-36. [CrossRef]
L. Labban, M. Kudsi, Z. Malek, N. Thallaj; Advances in Medical, Dental and Health Sciences, 2020,3, 3,45-48. [CrossRef]
L. Labban, N. Thallaj, M. Al Masri; Journal of Advanced Research in Food Science and Nutrition, 2020,3,1,34-41. [CrossRef]
Thallaj, N; agha, M. I ,H;, nattouf; A.H; katib, CH; karaali, A; Moustapha, A; Labban L;open access library journal, 2020,7,5,1-21. [CrossRef]
L. labban; N. thallaj; A. labban; archives of medicine, 2020, 12, 2:8, 1-5. [CrossRef]
L. labban; N. thallaj; international journal of herbal medicine,2020, 8, 2, 33-37.
L. Labban, N. Thallaj, Z. Malek; Journal of Medical Research and Health Sciences, 2019, 2, 11, 784-787.
L. labban N. Thallaj; acta scientific nutritional health, 2019, 3,10, 7- 12.
Malek, Z.S.; Sage D.; Pevet, P.; Raison, S.; Endocrinology 2007, 148 (11), 5165-5173. [CrossRef]
Malek, Z.S.; Dardente, H.; Pevet, P.; Raison, S.; European Journal of Neuroscience 2005, 22 (4), 895-901. [CrossRef]
Malek, Z.S.; Pevet, P.; Raison, S.; Neuroscience 2004, 125 (3), 749-758. [CrossRef]
Malek, Z.S.; Labban, L.; The International Journal of Neuroscience, 2020, 1-7.
Malek, Z.S.; Labban, L.; European Journal of Pharmaceutical and Medical Research 2019, 6 (11), 527-532.
Malek, Z.S.; Journal of AlBaath University 2018, 40 (4), 39-62.
Malek, Z.S.; Tishreen University Journal for Research and Scientific Studies, 2018, 40.
ZS Malek, LM Labban; International Journal of Neuroscience, 2021,131 (12), 1155-1161. [CrossRef]
ZS Malek, LM Labban; Journal of current research in physiology and pharmacology, 2020, 4, (1),1-5.
L.M. Labban, M. M. Alshishkli, A. Alkhalaf, Z. Malek; J. Adv. Res. Dent. Oral Health, 2017, 2(3&4), 1-4.
L Labban, ZS Malek, Open Access Library Journal, 2018, 5 (07), 1-11. [CrossRef]
L Labban, ZS Malek, Ann Food Nutr Res J, 2019,1 ,1
Labban, L. and N. Thallaj, 2019. Acta Scient. Nutr. Health, 3: 7-12. [CrossRef]